Many factors contribute to the diversity of life in an environment. The
availability of nutrients and sunlight, along with other factors that play a
pivotal role in determining what and how much life an area can sustain. While
studying the Second Law of Thermodynamics, it came to my attention that the
classical pyramid shape of the producer, C1, C2, C3, biomass pyramid did little
to take into account the amount of detrital input. I hypothesized that the
amount detrital input greatly effected the number of C1, C2, and C3 consumers
and thus the overall biodiversity of an ecosystem. Further, if you could find a
test-bed where detrital input was the only real difference between two similar
ecosystems you would find that organisms of each ecosystem would be adapted to
the peculiar conditions. This adaptation would lead you to find vast differences
in the taxonomic groups associated with each ecosystem. With this in mind, I
first set out to find two similar ecosystems were I could test this hypothesis.

Second, to sample, categorize and compare the diversity of these ecosystems
along taxonomic lines. Next, I planned to use several of the widely accepted
diversity indexes (Simpsonís Index, Shannonís Index the Chi-Square Test) to
compare statistically, the diversity of my ecosystems. Scientific Law states
that in order to test the effects of one factor in an equation you must
eliminate all other factors . In order to test the detrital base as the limiting
factor, all other limiting agents must be eliminated. In a field experiment this
is technically impossible; though it is possible to come close by choosing two
ecosystems that are very similar. In order to keep this experiment as simple as
possible the ecosystem chosen had to be nearly self contained and small. The
smaller and more contained the ecosystem the less chance for outside input that
could destroy our results. Alazan and Bernaldo creek provided just the type of
test-bed needed for this experiment. Both are third order creeks in the same
geographic area that are subject to same weather and climate conditions, but
differ considerably in the amount of detritus available. (Fleet) Procedure

Alazan creek is a third order stream that feeds into the Angelina River. It is
bordered by several species of indigenous trees that form a small gallery of
overhanging branches. This gallery consisted of (pine, oak, sweetgum trees) and
was limited to a range of about twenty five feet from the edge of the stream.

These gallery trees are surrounded by open cattle grazing fields covered by
short grasses and an occasional scrub brush. Alazan creek ranged from ten to
fifteen feet wide with a water depth of six inches to two feet. The water was
generally clear, and flowed at a brisk ten to twelve mile per hour pace. The
creek bottom was primarily sand with little or no mud. Turbitity was low to
moderately low and the creek had a high oxygen content. Detrital input was low
and limited to leaves from the gallery trees. Bernaldo creek is a third order
creek that similarly empties into the Angelina River. Bernaldo creek differs
substantially in that it is entirely surrounded by typical east Texas piney
woods. (The particular area that samples were taken from appeared to be
relatively low lying in comparison to the surrounding woods.) It is likewise ten
to fifteen feet wide but, is considerably deeper at four to eight feet than

Alazan creek. Bernaldo creek flows at a much slower pace, approximately six to
eight miles per hour. The bottom of Bernaldo creek consists largely of mud,
which gives the water a darker color. Overall turbitity is high and overall
oxygen content is low. Human disturbance at both creeks was minimal. Although at

Alazan creek the surrounding area was used for grazing animals and at Bernaldo
creek the sight that specimen were actually taken from was a concrete washout
bridge. Both sights appeared to be in a flood plain, one that probably becomes
inundated on a monthly basis during the rainy season. Weather conditions at the
time of the sampling were typical of east Texas in spring, therefore unusual
conditions caused by atypical weather can be eliminated. What it boils down to
is, the only difference between the two creeks was the amount of detrital
material available and the conditions predicated by this difference. Starting
the week of February 8, 1999 daily 1p.m. trips were made by four lab groups to
both Alazan and Bernaldo creeks. During these trips observations were made on
terrain, topography, climate, vegetation and specimens were taken from several
spots along each creek. The specimen were taken by netting at various depths and
locations. The nets used had a pore size of approximately 2 millimeters on four
sides and a canvas bottom (see diagram 1) and were attached to poles 8 feet
long. In order to take a sample, a student placed the scoop nets open end up
stream and allowed the water and itís contents to be strained. The nets were
then quickly pulled from the water and the samples collected were immediately
taken to opened garbage bags and sorted through. (see diagram 2) When any living
creature was found, it was placed in a collection jar (labeled for the
particular creek it was taken from) to be examined later. The collection jars
contained an organic die known as FAA. FAA is a combination of formalin, ethyl
alcohol, and Rose Bengal and tints most of the small "bugs" a red/pink
color. The following week each lab examined the specimen jars one by one and
separated the contents by taxonomic groups. Once each creekís specimens had
been counted and categorized by class period, a list was compiled for the weeks
totals. This list was then used to test by comparison the validity of our
hypothesis. (for the complete list and breakdown see chart 1) Results The
hypothesis I was attempting to prove had three parts. The first and most general
was the creek with the greater detrital base would have greater biodiversity.

This can be proven in several ways. The first is to simply count the number of
species present in each of the two creeks and compare the results. This is
called richness, which is the number of species/taxonomic groups. In that case

Alazan creek contained 13 species/ taxomic groups and Bernaldo creek had 17.

Therefore, Bernaldo creek which had the greater detrital base had 4 more species
than Alazan creek. A second part of counting species is to determine the
evenness of the the creeks. Evenness is the measure of how evenly divided the
individuals are among the taxonomic groups. Bernaldo creek had Next I used
several of the accepted diversity indexes to statically prove which creek had
the greater diversity. Simpsonís Index is the number of times it would take to
pick two individuals of the same species/taxonomic group. Simpsonís index is
calculated by the equation: D = {N(N-1)} / {En(n-1)} Where: N=Total number of
species/taxonomic groups n=Number of individuals of a species. (Cox) In this
case Bernaldo creek had a Simpsonís Index of .017712946 and Alazan creek had a

Simpsonís Index of .0092367032. Thatís a difference of .0084762429, or a 91
% greater chance of getting two of the same organisms. This shows a
significantly greater level of diversity for Bernaldo creek than for Alazan.

Shannonís Index in determined by the equation: Hí=3.3219[log N - 1/N E(Ni
log Ni)] With "N" being the total number of individuals in the sample,

"Ni" being the number of individuals in each species/taxonomic group, and

"E" being the summation of all logs. (Cox) Bernaldo creek had a Shannons

Index of 2349.0908. Alazan creek had a Shannonís Index of 1876.1630. Thatís
a difference of 473.9278, or approximately 40%. The proves that the diversity in

Bernaldo creek is higher than the diversity of Alazan creek. "The null
hypothesis that the two Shannon diversity indices come from communities equal in
diversity can be tested by a test a "t" test." This test is used to
calculate chance of a type one error. The equation for this is: t = (H1 - H2) /

Sd The "t" value for the above was 3.290 significantly within our accepted
margin of error .0005. (t=3,290P,.0005) Next, the Chi-Square test was performed,
itís information is given by the equation: X^2=E{(observed-expected)} /
expected Where: expected value is given by {(row total x column total) / grand
total} and "E" is the summation. (Cox) In this case (X^2 = , P,.005)

Discussion The evidence collected in our study significantly proves the
hypothesis. All of the confidence intervals were met and exceeded in each test
with out exception. This was also the case with each of my peers that performed
this experiment. Although this increases the general knowledge on the impact of
detrital input into a system there is more to be learned. There were three main
sources of potential errors in this experiment. First, there needed to be more
samples taken from more places and at different times of the year. Four
samplings from one spot on the creek are not enough to draw conclusions about
the entire system. What if the area tested was near some source of point
pollution? This could have an effect on the immediate area but cause no down
stream effects due to rapid break down, or simple dilution. What if the area
picked was more diverse during summer? Next, an unforeseen problem occurred when
the crayfish began eating many of the "bugs" in the collection jars. This
caused many of our species/taxonomic groups to be under represented because they
got eaten before they could be counted! What about animals that were so small
they slipped through the holes in the nets? What about burrowing worms? None of
these animals are represented in the sampling. Had these species/groups been
represented some of the statistics might have been a little different. In the
future this test should be modified in a manner to correct some of the
afore-mentioned problems. With those modifications a person could build an even
stronger case to support the hypothesis.